Effects of NaHS on MBP and learning and memory in hippocampus of mice with spinocerebellar ataxia / 中国应用生理学杂志

To investigate the effects of exogenous NaHS on myelin basic protein (MBP) and learning and memory of hippocampal neurons in mice with spinocerebellar ataxia type 3 (SCA3) and its therapeutic significance. Twelve male normal mice were randomly selected as normal control group (NC Group), and 48 SCA3 mice were randomly selected as SCA3 model group (M Group), low dose group (NL Group, 10 μmol/kg), medium dose group (NM Group, 50μmol/kg) and high dose group (NH Group, 100 μmol/kg), 12 rats in each group. The drug treated groups were injected with NaHS intraperitoneally once a day for 4 weeks. The changes of learning and memory ability of SCA3 mice before and after the intervention of different doses of NaHS were determined by Morris water maze, the content of hydrogen sulfide (HS) in hippocampus was measured by spectrophotometry, the expression of MBP was detected by immunohistochemistry, and the morphological changes of neuron myelin sheath were observed by electron microscope. Compared with the control group, the learning and memory ability of SCA3 mice was decreased significantly (P＜0.05), and the content of HS in hippocampus was decreased (P＜0.05). After different doses of exogenous NaHS treatment, the learning and memory ability was improved in different degrees (P＜0.05), and the contents of HS and MBP in hippocampus of SCA3 mice were also improved in different degrees (P＜0.05). Exogenous NaHS may increase the contents of HS and MBP in the hippocampus of SCA3 mice, which may have a protective effect on the neurons, and then improve the learning and memory ability of SCA3 mice, and provide a new idea for the treatment of SCA3.

Impact of childhood sexual abuse on risky sexual behaviors among men who have sex with men in Guangzhou / 中华疾病控制杂志

Objective To explore the experience of childhood sexual abuse (CSA) among men who have sex with men (MSM) in Guangzhou, and to evaluate its impact on risky sexual behaviors in adulthood. Methods A non-probability sampling method was used to recruit MSM aged 18 years and over in Guangzhou. Sociodemographic characteristics, experience of CSA and risky sexual behaviors during the past 6 months were collected using an electronic questionnaire. 2 tests were used to compare the prevalence of CSA and risky sexual behaviors during the past 6 months among different sociodemographic characteristics. Logistic regressions were performed to assess the impact of CSA on risky sexual behaviors. Results Overall, 976 MSM were included with an average age of (28.35±6.83) years old. Nearly one-third (26.95%, 263/976) of MSM experienced CSA. Participants having multiple sexual partners (MSP) and unprotected anal intercourse (UAI) during the past 6 months accounted for 61.68% (602/976) and 47.95% (468/976), respectively. After adjusting age, ethnicity, household registration, local residence time, marital status, education, employment status, monthly income and sexual orientation, the results of multivariate Logistic regression showed that MSM who experienced CSA were likely to have MSP during the past 6 months (aOR=1.62; 95%CI: 1.19-2.22) than those did not experience CSA. But the association between UAI and CSA was not statistically significant. Conclusions The prevalence of CSA among MSM in Guangzhou is high. CSA can substantially increase the risk of risky sexual behaviors in adulthood. Intervention to reduce the risk of sexual risk behaviors may need to be tailored for MSM who experienced CSA.

Association between sexual sensation seeking and sexual risk behaviors among men who have sex with men in Guangzhou / 中华疾病控制杂志

Objective To evaluate the association between sexual sensation seeking (SSS) and sexual risk behaviors among men who have sex with men (MSM) in Guangzhou. Methods MSM aged 18 and over were recruited in Zhitong Charity from June 2017 to April 2018. Data on socio-demographic characteristics, sexual behaviors and SSS were collected using an electronic questionnaire. Logistic regression analyses were performed to evaluate the association between SSS and sexual risk behaviors. Results A total of 500 MSM were included in the current study. The mean age of the participants was (27.77±5.96) years old. The multiple logistic regression analyses showed that MSM with high SSS scores were more likely to engage in multiple sexual partners (OR=2.05, 95% CI: 1.40-2.30,P<0.001), one-night stand (OR=2.11, 95% CI: 1.44-3.09,P<0.001), and unprotected anal intercourse (OR=1.70, 95% CI: 1.16-2.50,P=0.007) than those with low SSS scores. Conclusions High SSS is associated with an increased risk of sexual risk behavior among MSM in Guangzhou. The behavior intervention strategy should be tailored for MSM with high SSS to improve the effectiveness to reduce sexual risk behaviors.

Analysis of pathogenic spectrum and clinical characteristics of viral diarrhea in children / 中国实用儿科杂志

OBJECTIVE: To study the pathogenic spectrum and the clinical characteristics of viral diarrhea in children.METHODS: The study was conducted in 400 children with viral diarrhea hospitalized in Children's Hospital from January to December in 2016. The stool specimens(about 5 mL)were collected for detection from the children on the day of hospitalization. The ELISA method and PCR method were used to detect the genotypes of HUCV,HADV,HAsta V and HRV in stool specimens,and the clinical data of children were collected at the same time. RESULTS: In 2016,the total positive rate of HRV,HUCV,EAd V and HAst V in the stool specimens of children with viral diarrhea was 59%(236 cases). The positive detection rates of the four viruses were 178 cases(75.42%),68 cases(28.81%),12 cases(5.08%)and 5 cases(2.12%)respectively,of which 11.42% were double mixed infection and 41% were of unknown pathogens. The HRVG6(93.63%,147 cases)and P3(91.08%)as well as G6[P3](90.45%)combination were the popular superior type in 2016,and about 11.79%couldn't be typed. Norovirus accounted for 92.65% of the calicivirus viruses,and GⅡ accounted for 98.41%. About 81.79%of the children(193 cases)hospitalized for viral diarrhea were less than 24 months old. Pathogens were different at different peak age. The peak season of onset was fromJanuary to March and December. The main clinical manifestations were diarrhea,vomiting and fever. CONCLUSION: It is essential to determine the unknown pathogens and improve the pathogenic spectrum in children with viral diarrhea. Viral diarrhea is often accompanied by extraintestinal clinical manifestations.

Effects of aminooxyacetic acid on the learning and memory ability and its possible mechanism in rats with chronic alcoholism / 中国应用生理学杂志

OBJECTIVE@#To investigate the effects of aminooxyacetic acid (AOAA) on learning and memory ability and possible mechanisms in rats with chronic alcoholism.@*METHODS@#Sixty SD male rats were randomly divided into three groups on average.The model group rats and the remedy group rats were fed with the water containing (v/v) 6% alcohol for 28 days.After 14 days, the remedy group rats were treated with AOAA (5 mg/kg·d) by intraperitoneal injection once a day for 14 days and the other two group rats were treated with the equal amount of saline by intraperitoneal injection every day.Five days before the end of the experiment, the water maze test was carried out to test the learning and memory ability of rats for 5 days.Subsequently, the content of HS, the activity of ATP enzyme and the expression of 5-HT in hippocampus were measured.@*RESULTS@#Compared with the rats in the control group, the latency and the swimming distance of the 2nd to the 4th day, the content of HS in hippocampus of rats in the model group were all increased, the mitochondrial ATP enzyme activity in hippocampus and the positive expression of 5-HT in hippocampus CA1 and CA3 of rats in the model group were decreased (<0.01).Compared with the rats in the model group, the latency and the swimming distance of the 2nd to the 4th day, the content of HS in hippocampus of the rats in the remedy group were decreased, the mitochondrial ATP enzyme activity in hippocampus and the positive expression of 5-HT in hippocampus CA1 and CA3 of rats in the model group were increased (<0.01).@*CONCLUSIONS@#AOAA could alleviate the symptoms of chronic alcoholism rats, which may be related to the effects of AOAA on the content of HS, the mitochondrial enzyme activity and the expression of 5-HT in hippocampus.

Depression and associated factors among migrant women of reproductive age in some areas of Wuhan / 中华流行病学杂志

<p><b>OBJECTIVE</b>To understand factors as social support, situation of depression and related risk factors among migrant women of reproductive age in some areas of Wuhan.</p><p><b>METHODS</b>Cluster sampling method and face-to-face interview with structured questionnaire were used to investigate 316 migrant women in Wuhan.</p><p><b>RESULTS</b>The overall prevalence rate of depression was 32.3%.</p><p><b>RESULTS</b>from binary logistic regression analysis showed that factors as: under pressure (OR = 4.010, 95%CI: 1.672 - 9.617), having had negative life events (OR = 2.050, 95%CI: 1.170 - 3.591) or tight relations with neighbors (OR = 2.537, 95%CI: 1.053 - 6.113), not quite satisfied (OR = 4.247, 95%CI: 1.521 - 11.855) or satisfied (OR = 2.411, 95%CI: 1.111 - 5.233), on his/her own health status etc., might serve as the possible risk factors for depression. In scores related to social support, and the utilization of such support, there were statistically significant differences seen between the groups with depression and the one without.</p><p><b>CONCLUSION</b>The prevalence of depression among migrant women of reproductive age appeared to be high in some areas on Wuhan. Factors as: under pressure, having had negative life events, tight relations with neighbors, status on satisfaction of one's own health situation as well as related social support, seemed to be the main risk factors for depression in this population.</p>

Recent advances in the study of natural homoisoflavonoids / 药学学报

Homoisoflavonoid is a special type in flavonoids. There are more than 110 homoisoflavonoid compounds isolated from natural materials. Homoisoflavonoid compounds show many bioactivities on anti-inflammatory, estrogenicy, antiestrogenic, anticancer and angioprotective etc. This paper summarized the plant sources, structural types spectrocopy features and the biology activities of homoisoflavonoids.

Effect of Progesterone on Expressions of Superoxide Dismutase and Matrix Metalloproteinase-3 in Brain Tissues of Neonatal Rats with Hypoxic-Ischemic Brain Damage / 实用儿科临床杂志

Objective To explore the expressions of superoxide dismutase(SOD)and matrix metalloproteinase-3(MMP-3) in the brain tissues of newborn rat after hypoxic-ischemic(HI) and the effects of progesterone(PROG) on SOD and MMP-3 expression.Methods Seventy-two neonatal SD rats were randomly divided into 4 groups:normal control group(normal group),sham-operated group,HI group and PROG group.The model of neonatal rat with hypoxic-ischemic brain damage(HIBD) was made.The permeability of the blood-brain barrier was observed by using transmission electron microscopy,the level of SOD in the brain was assessed by hydroxylammoniumchloride autoxidation,MMP-3 protein expression in cerebral cortex part of the brain was detected by immunohistochemical.SPSS 12.0 software was used to analyze the data.Results In normal group and sham-operated group,capillary vessel′s morphology of blood brain barrier of neonatal rats were integrity and smooth,and structure were clarity.The permeability of blood-brain barrier in HI group was obviously enhanced and had slight changes in PROG group.Compared with the sham-operated group,the level of SOD in HI group significantly decreased(P